Journal of Aquaculture Research & Development

Won-gyu Park

Publications

• Research Article
  cDNAs Encoding Chitin Synthase from Shrimp (Pandalopsis Japonica): Molecular Characterization and Expression Analysis
  Author(s): Md. Hasan Uddowla,Ah Ran Kim,Won-gyu Park,Hyun-Woo Kim*Md. Hasan Uddowla,Ah Ran Kim,Won-gyu Park,Hyun-Woo Kim*
  
  Crustacean growth occurs via molting, the periodic shedding of the exoskeleton. Understanding the genes involved in chitin metabolism associated with the periodic molt cycle is important for various applications to decapod crustacean aquaculture. Chitin synthase is an important enzyme in the chitin biosynthetic pathway that plays a major role in synthesis of new cuticle after molting. In this study, we isolated a full-length cDNA encoding chitin synthase (PajCHS) from Pandalopsis japonica through a combination of PCR (Polymerase chain reaction)- based cloning and bioinformatics analysis. The identified PajCHS encodes a transmembrane protein with 1525 amino acid residues (175 kDa). Comparison with other CHSs from insects revealed that PajCHS contains three domains: N-terminal domain A, catalytic domain B, and C-terminal domain C. Three conserved motifs (EDR, QRRRW, and SWGTR) were also.. View More»
  DOI: 10.4172/2155-9546.1000298

  Abstract PDF