Journal of Blood Disorders & Transfusion

Wojciech Krzyzanski

Publications

• Research Article
  Dynamics of Erythropoietic Markers in Response to Hypoxia in Rats
  Author(s): Ying Zhang, Indrajeet Singh and Wojciech Krzyzanski Ying Zhang, Indrajeet Singh and Wojciech Krzyzanski
  
  Purpose: Erythropoietin (EPO) regulates production of red blood cells in response to hypoxia. Decreased arterial oxygen saturation results in an increased production of EPO in various tissues including kidney as a major stimulation site leading to an increase in EPO plasma levels. This study aims to investigate the relationship between hypoxia and resultant erythropoietic response in rats. Methods: The in house hypoxia chamber was designed to regulate the fraction of inspired oxygen (FiO2) = 10% supplied to Wistar male rats (N=4) for 6 hours by mixing room air with N2 to maintain air pressure at 1 atm. Plasma and kidney samples were collected at various times during and after exposure to hypoxia. Kidney EPO mRNA, HIF-2α, plasma EPO concentration, reticulocyte counts and hemoglobin were measured. The time course of above markers and fold changes from pre-hypoxia va.. View More»
  DOI: 10.4172/2155-9864.1000197

  Abstract PDF