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Warwick E
Warwick E
Australia
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Research Article
Challenges in Analysis of Circulating Extracellular Vesicles in Human Plasma Using Nanotracking and Tunable Resistive Pulse Sensing
Author(s): Enjeti AK, Ariyarajah A, Warwick E, Seldon M and Lincz LF Enjeti AK, Ariyarajah A, Warwick E, Seldon M and Lincz LF
Aim: Extracellular vesicles (EV) are secreted from both healthy and diseased tissues and are detectable in most body fluids, where their measurement can be of prognostic/diagnostic value. We aimed to evaluate pre-analytical and analytical variables in the measurement of EV in human plasma using nanotracking analysis (NTA) and tunable resistive pulse sensing (TRPS). Method: Commercial beads (200 nm and 400 nm in diameter) and human plasma from volunteer donors were used in this study. A total of 36 bead and 175 plasma measurements were undertaken by NTA and TRPS. The preand analytical conditions tested on plasma samples were: fresh, stored for 1 day or 1 week at 4°C or -80°C. The other variables included testing of neat or pelleted EVs and choice of diluent. Results: The 200 nm and 400 nm beads, when tested alone .. View More»
DOI: 10.4172/2157-7439.1000468