Journal of Petroleum & Environmental Biotechnology

Wael S El- Sayed

Publications

• Research Article
  Bacterial Degradation of the Saturate Fraction of Arabian Light Crude oil: Biosurfactant Production and the Effect of ZnO Nanoparticles
  Author(s): Wael Ismail, Noora A Alhamad, Wael S El-Sayed, Ashraf M El Nayal, Yin-Ru Chiang, Riyad Y HamzahWael Ismail, Noora A Alhamad, Wael S El-Sayed, Ashraf M El Nayal, Yin-Ru Chiang, Riyad Y Hamzah
  
  The aim of this research was to study the influence of nanoparticles and the initial oil amount on the biodegradation of crude oil. Production of biosurfactants was also assessed. Crude oil-utilizing bacteria were isolated from oilfields via enrichment in chemically defined medium with crude oil a sole carbon source. The isolates could be affiliated to the genera Bacillus, Pseudomonas, Achromobacter, and Microbacterium by 16S rDNA sequencing and phylogenetic analysis. GC/FID analysis revealed 52 to 98% degradation of the oil saturate fraction within one month. Nanoparticles of ZnO inhibited growth and crude oil biodegradation by one isolate. (NBHCO4). Two strains, NBHCO2 and NCEOW, emulsified and utilized water-in-oil emulsions (chocolate mousse). Two bacterial strains, I-19 and NBHCO2 grew with crude oil in cultures containing up to 20% oil. Degradation extent in the I-19 culture inc.. View More»
  DOI: 10.4172/2157-7463.1000163

  Abstract PDF