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Vida Mahinpo
Vida Mahinpo
West Indies
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Research Article
Investigation of Fusarium verticillioides on the Basis of RAPD Analysis, and Vegetative Compatibility in Iran
Author(s): Zeynab Bahmani, Reza Farokhi Nejad, Khoshnood Nourollahi, Fatemeh Fayazi and Vida MahinpoZeynab Bahmani, Reza Farokhi Nejad, Khoshnood Nourollahi, Fatemeh Fayazi and Vida Mahinpo
Genetic diversity among 41 isolates was determined using vegetative compatibility groups from different regions of Fars (Firozabad, Ramjerd, Drodzan, Shiraz, Pasargad, Sepidan and Mravdasht) and Khuzestan (Ezh, Ramhormoz and Ahodasht) provinces. Nitrate non-utilizing (Nit) mutants were generated on Czapeck media, containing 5% KClO3. Phenotypic classes of nit mutants were determined to 17 different Vegetative Compatibility Groups (VCGs) on media containing nitrate, hypoxanthine and ammonium. The largest group contained 18 isolates and others contained two and one, respectively. 24 isolates were selected for RAPD test, to represent 17 vegetative compatibility groups. A set of seven random primers revealed a total of 36 alleles. High level of genetic diversity was observed among F. verticillioides isolated in the region. 29 alleles (80.55) showed high polymorphism in all the isol.. View More»
DOI: 10.4172/2157-7471.1000147