Journal of Bioequivalence & Bioavailability

Valerii E Sabko

Publications

• Research Article
  UPLC-MS/MS Quantification of Fenspiride in Human Plasma
  Author(s): Yuriy V Pidpruzhnykov, Valerii E Sabko, Volodymyr V Iurchenko and Igor A ZupanetsYuriy V Pidpruzhnykov, Valerii E Sabko, Volodymyr V Iurchenko and Igor A Zupanets
  
  A new simple and rapid ultra-performance liquid chromatography - tandem mass spectrometry (UPLC-MS/ MS) method has been developed and validated for the quantification of fenspiride in human plasma. Following a simple protein precipitation with acetonitrile, the analyte and internal standard (bupivacaine) were separated on an ACQUITY UPLC ® BEH С18 column (50 mm×2.1 mm, 1.7 μm) using a gradient mixture of acetonitrile and water (both containing 0.2% formic acid) as a mobile phase at flow rate 0.4 mL/min. The retention time of fenspiride and bupivacaine was 0.64 and 0.87 min, respectively. Detection was operated in positive mode by monitoring the transitions of m/z 261→105 for fenspiride and m/z 289→140 for bupivacaine. The lower limit of quantification was 2 ng/mL by using 200 μL aliquots of plasma. The range of quantification of fenspiride was 2-500 ng/mL. W.. View More»
  DOI: 10.4172/jbb.1000156

  Abstract PDF