Utkan G
Turkey
Research Article
Moderately Halophilic Bacterium Halomonas sp. AAD12: A Promising Candidate as a Hydroxyectoine Producer
Author(s): Öztürk HU, Sariyar Akbulut B, Ayan B, Poli A, Denizci AA, Utkan G, Nicolaus B and Kazan DÖztürk HU, Sariyar Akbulut B, Ayan B, Poli A, Denizci AA, Utkan G, Nicolaus B and Kazan D
Properties of osmolytes that help protect biological molecules from stress and preserve their functions impose significant importance to finding new organisms with different osmolyte accumulation strategies. To this end, the moderately halophilic Halomonas sp. AAD12 has been characterized for its adaptation to stress conditions with particular emphasis on its osmolyte accumulation strategy. The effect of temperature, salinity, aeration and organic components on the accumulation of osmoprotectants and synthesis of fatty acids were examined in M63 minimal medium. Ectoine, proline and hydroxyectoine were the major osmolytes and palmitic acid (16:0), palmitoleic acid (16:1), and oleic acid (18:1) were the major fatty acids. Overall, ectoine yield was the highest among the three osmolytes at all salt concentrations and temperatures investigated. However, high salinity reduced ectoine yield.. View More»
DOI:
10.4172/1948-5948.1000222