Journal of Stem Cell Research & Therapy

Toshinori Ito

Publications

• Research Article
  Murine Insulinoma Cell-Conditioned Medium with ΒETA2/Neurod1 Transduction Efficiently Induces the Differentiation of Adipose-Derived Mesenchymal Stem Cells into β-Like Cells both In Vitro and In Vivo
  Author(s): Koichi Kawamoto, Shigeharu Yabe, Masamitsu Konno, Hideshi Ishii, Naohiro Nishida, Jun Koseki, Satsuki Fukuda, Yoshito Tomimaru, Naoki Hama, Hiroshi Wada, Shogo Kobayashi, Hidetoshi Eguchi, Masahiro Tanemura, Toshinori Ito, Eun Young Lee, Eri Mukai, Takashi Miki, Yuichiro Doki, Masaki Mori, Tatsuo S Hamazaki, Hiroaki Nagano and Hitoshi Okochi Koichi Kawamoto, Shigeharu Yabe, Masamitsu Konno, Hideshi Ishii, Naohiro Nishida, Jun Koseki, Satsuki Fukuda, Yoshito Tomimaru, Naoki Hama, Hiroshi Wada, Shogo Kobayashi, Hidetoshi Eguchi, Masahiro Tanemura, Toshinori Ito, Eun Young Lee, Eri Mukai, Takashi Miki, Yuichiro Doki, Masaki Mori, Tatsuo S Hamazaki, Hiroaki Nagano and Hitoshi Okochi
  
  Background: Mesenchymal stem cells (MSCs), including adipose tissue-derived mesenchymal stem cells (ADSCs), are multipotent and can differentiate into various cell types, including pancreatic β cells. Therefore, ADSCs present a potential cell source for the treatment of type 1 diabetes mellitus (T1DM). However, current in vitro protocols are insufficient to induce fully matured insulin-producing β cells. In this study, we assessed the effectiveness of overexpression of ΒETA2 (NeuroD1), a member of the basic helix–loop–helix transcription factor family, with murine insulinoma cell line-derived conditioned medium (MIN6-CM) to improve the differentiation capacity of ADSCs into insulin-producing cells. Method: Murine ADSCs were isolated from C57BL/6 mice, transduced with several transcrip.. View More»
  DOI: 10.4172/2157-7633.1000221

  Abstract PDF