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Tom Edlind
Tom Edlind
Tanzania
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Research Article
Development and Evaluation of a Commercial Sequence-Based Strain Typing Service for Listeria monocytogenes
Author(s): Tom Edlind and Yanhong Liu Tom Edlind and Yanhong Liu
Listeria monocytogenes is an important foodborne pathogen and, relatedly, a persistent contaminant in many food processing facilities. Strain typing is critical to detecting and investigating outbreaks, and can be used to track down sources of contamination. The typing systems in current use have limitations ranging from low resolution and data portability to high cost and technical complexity. The aim of this study was to develop an outsourcing option for L. monocytogenes strain typing that addresses these limitations. The NCBI Genomes database representing 109 strains was screened for highly informative, tandem repeat-containing loci. The most promising, termed LmMT1 (0.8-1 kbp) and LmMT2 (0.7-0.8 kbp), exhibited complex patterns of polymorphism (insertions/deletions and single nucleotide polymorphisms), diversity indeces of 0.99 (LmMT1) and 0.98 (LmMT2), and were present in all L. .. View More»
DOI: 10.4172/1948-5948.1000238