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Thomas Lubberstedt
Thomas Lubberstedt
Tanzania
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Research Article
Unraveling the Genetic Diversity of Maize Downy Mildew in Indonesia
Author(s): Rudy Lukman, Ahmad Afifuddin and Thomas LubberstedtRudy Lukman, Ahmad Afifuddin and Thomas Lubberstedt
Varying effectiveness of metalaxyl fungicides and disease incidences caused by downy mildew to maize in several places in Indonesia led to the speculation that genetic variation of Peronosclerospora species in Indonesia exists. Hence, we employed two molecular marker systems, namely SSR (Simple Sequence Repeat) and ARDRA (Amplified Ribosomal DNA Restriction Analysis) markers, to study the population structure and genetic diversity of downy mildew isolates collected from hotspot production areas of maize in Indonesia. Both molecular techniques grouped the isolates into three clusters with a genetic similarity between 66-98% and 58-100% for SSR and ARDRA markers, respectively. In general, SSRs yielded lower similarities among isolates compared to ARDRA. Combined analysis of data from both techniques resulted in genetic similarities of 64-98% for 31 downy mildew isolates grouped into thr.. View More»
DOI: 10.4172/2157-7471.1000162