Thomas Albert Miller
Tanzania
Research Article
Endophytic Bacteria Associated to Sharpshooters (Hemiptera:
Cicadellidae), Insect Vectors of Xylella fastidiosa Subsp. pauca
Author(s): Cláudia Santos Gai, Francisco Dini-Andreote1, Fernando Dini Andreote, João Roberto Spotti Lopes, Thomas Albert Miller, João Lúcio Azevedo and Paulo Teixeira LacavaCláudia Santos Gai, Francisco Dini-Andreote1, Fernando Dini Andreote, João Roberto Spotti Lopes, Thomas Albert Miller, João Lúcio Azevedo and Paulo Teixeira Lacava
Xylella fastidiosa subsp. pauca causes citrus variegated chlorosis (CVC) disease in Brazil, resulting in significant production losses in the citrus industry. X. fastidiosa subsp. pauca is mainly transmitted by three species of sharpshooters (Hemiptera: Cicadellidae) in Brazil; Dilobopterus costalimai (Young), Acrogonia citrina Marucci & Cavichioli and Oncometopia facialis (Signoret). We identified bacterial communities associated with the heads of surface-sterilized insect vectors of X. fastidiosa subsp. pauca that were collected from CVC affected citrus groves in Brazil. Bacteria were isolated and analyzed by amplified ribosomal DNA restriction analysis (ARDRA) and sequencing, revealing the presence, among the most abundant genera, of the well-known citrus endophytes Methylobacterium spp. and Curtobacterium spp. Specific PCR systems for the detection of these genera indicated hi.. View More»
DOI:
10.4172/2157-7471.1000109