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Tek Chand Bhalla
Tek Chand Bhalla
Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla,
India
Publications
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Research Article
Statistical Enhancement of Cyanide Degradation Using Microbial Consortium
Author(s): Virender Kumar, Vijay Kumar and Tek Chand BhallaVirender Kumar, Vijay Kumar and Tek Chand Bhalla
Remediation of cyanide contaminated water bodies using microorganisms is a popular alternative over chemical and physical methods of cyanide detoxification. The objective of the present study is to develop a microbial consortium using three bacteria, i.e., Enterobacter sp. RL2a, Serratia marcescencs RL2b and Achromobacter sp. RL2c for effective degradation of simulated cyanide wastewater. In vitro cyanide degradation was optimum with 2% inoculum volume of cells; pH 6.0, 30°C temperature at 20 mM substrate concentration leading to complete cyanide removal in 36 h. Response surface methodology (RSM) approach was used for optimization of reaction conditions for cyanide degradation using 5 mg ml-1 resting cells of microbial consortium. Plackett-burman design depicted that three variables viz. time, resting cells of strain RL2b and pH exhibit positive effect on cyanide degradation. The.. View More»
DOI: 10.4172/1948-5948.1000237