Biochemistry & Analytical Biochemistry

Tanuja Kosuri

Publications

• Research Article
  Rapid Detection of Proteins on One-Dimensional Polyacrylamide Gel Electrophoresis Using an Inexpensive Fluorescent Optical Brightener
  Author(s): Sashidhar RB, Tanuja Kosuri and Sujata Nayak Sashidhar RB, Tanuja Kosuri and Sujata Nayak
  
  A commercial fabric fluorescent optical brightener Ranipal® (F-OB), has been successfully employed to stain proteins on native and SDS-1D-PAGE. The F-OB was purified by using a biphasic solvent system of dichloromethane and water. The Rf value (0.63) of purified and crude F-OB were comparable on TLC. The mass spectrometry of purified F-OB indicated base peak at 414 (m/z). Absorption and emission maxima of F-OB were found to be 350 nm and 430 nm, respectively. The F-OB could stain the proteins both pre- and post-electrophoretic run, on native gel. Postelectrophoretic staining was rapid and required 20 min to visualize the stained proteins. On the other hand, in SDS gels, an additional 20 min was required for the extraction of SDS before staining the proteins with F-OB. SDS was found to interfere with binding of F-OB to protein.. View More»
  DOI: 10.4172/2161-1009.1000293

  Abstract PDF