Biochemistry & Analytical Biochemistry

Swati Singh

Publications

• Research Article
  A Defense Associated Peroxidase from Lemon Having Dye Decolorizing Ability and Offering Resistance to Heat, Heavy Metals and Organic Solvents
  Author(s): Veda P Pandey, Prakash K Bhagat, Ramgopal Prajapati, Nivedita Jaiswal, Swati Singh, Manika Awasthi and Upendra N Dwivedi Veda P Pandey, Prakash K Bhagat, Ramgopal Prajapati, Nivedita Jaiswal, Swati Singh, Manika Awasthi and Upendra N Dwivedi
  
  Peroxidases have ability to catalyze redox reaction of a wide range of phenolic as well as non-phenolic compounds and exhibited various physiological roles in plant life cycle. From the point of view of industrial applications of peroxidases, the isolation and characterization of peroxidases offering resistance to higher pH and temperature, salts, organic solvents is highly desirable. In this direction, a partial cDNA clone of peroxidase from lemon was isolated and characterized. The peroxidase was found to be defense associated as evident by the higher expression in diseased condition than that of healthy one at both the transcript and enzymatic activity levels. This defense related peroxidase from lemon leaves was purified to homogeneity using quick two step processes of heat treatment and affinity chromatography. The native peroxid.. View More»
  DOI: 10.4172/2161-1009.1000291

  Abstract PDF