Journal of Blood Disorders & Transfusion

Svetla Stoilova-McPhie

Publications

• Research Article
  Binding of Factor VIII to Lipid Nanodiscs Increases its Clotting Function in a Mouse Model of Hemophilia A
  Author(s): Keri Csencsits-Smith, Krill Grushin and Svetla Stoilova-McPhieKeri Csencsits-Smith, Krill Grushin and Svetla Stoilova-McPhie
  
  Background: Hemophilia A is a congenital bleeding disorder caused by defective or deficient factor VIII (FVIII). The active form of FVIII is the co-factor for the serine protease factor IXa (FIXa) in the membrane-bound intrinsic tenase (FVIIIa-FIXa) complex. The assembly of the FVIIIa-FIXa complex on the activated platelet surface is critical for successful blood clotting. Objectives: To characterize the role of lipid nanodiscs (ND) for on FVIII function in vivo and test the lipid ND as a delivery system for FVIII. To evaluate the potential of binding recombinant FVIII to ND as improved treatment for Hemophilia A. Methods: Recombinant porcine FVIII (rpFVIII) was expressed and characterized in solution, and when bound to ND. The rpFVIII, ND and rpFVIII-ND complexes were characterized via transmission electron microscopy. Functional studies were carried out using aPTT te.. View More»
  DOI: 10.4172/2155-9864.1000325

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