Journal of Aquaculture Research & Development

Subhasha Nigam

Publications

• Research Article
  Proteomic Analysis of Sensitive and Resistant Isolates of Escherichia coli in Understanding Target(s) of a Cyanobacterial Biomolecule Hapalindole-T
  Author(s): Manoj Kumar Tripathi, Maheep Kumar, Deepali S, Ravi Kumar Asthana and Subhasha Nigam Manoj Kumar Tripathi, Maheep Kumar, Deepali S, Ravi Kumar Asthana and Subhasha Nigam
  
  A broad spectrum biomolecule Hapalindote-T, from a cyanobacterium Fischerella sp. colonizing Neem tree bark was used for its targets using Escherichia coli. The cellular extracts of Hap-TS (sensitive) and Hap-TR (resistant) of E. coli were subjected to 2DGE. The protein spots (selected) with altered expression were analysed by LC-MS. The data obtained was matched with database of E. coli. Seventeen proteins were found with altered expression level. Three membrane proteins, OmpP, Agn43A and LysU, found in Hap-TS strain were absent in the Hap-TR strain. However, fourteen proteins, AspA, GlpK, LpdA, HslU, GlnA, SucB, YihT, GalF, MDH, RfbB, RmlB, AcrAB, FabB and GapA, related to certain metabolic pathways of the cell and overproduced in the extract of Hap-TR strain. The seventeen screened proteins were related with vital metabolic pathways including membrane protein (Omp P), in E. coli. T.. View More»
  DOI: 10.4172/2155-9546.1000467

  Abstract PDF