Journal of Carcinogenesis & Mutagenesis

Subhash C Gautam

Publications

• Research Article
  Pristimerin Induces Apoptosis in Prostate Cancer Cells by Downregulating Bcl-2 through ROS-dependent Ubiquitin-proteasomal Degradation Pathway
  Author(s): Yong Bo Liu, Xiaohua Gao, Dorrah Deeb, Ali S Arbab and Subhash C GautamYong Bo Liu, Xiaohua Gao, Dorrah Deeb, Ali S Arbab and Subhash C Gautam
  
  Pristimerin is a quinonemethide triterpenoid with the potential of a promising anticancer agent. Pristimerin (PM) has shown anticancer activity against a range of cancer cell lines, but its activity for prostate cancer has not been adequately investigated. In the present study we have examined the underlying mechanisms of the apoptotic response of the hormone-sensitive (LNCaP) and hormone-refractory (PC-3) prostate cancer cell lines to PM. Treatment with PM induced apoptosis in both cell lines as characterized by increased annexin V-binding and cleavage of PARP-1 and procaspases-3 and -9. It also induced mitochondrial depolarization, cytochrome c release from mitochondria and generation of reactive oxygen species (ROS). Response to PM is regulated by Bcl-2 since it down-regulated Bcl-2 expression and overexpression of Bcl-2 rendered prostate cancer cells resistant to PM. ROS plays a r.. View More»
  DOI: 10.4172/2157-2518.S6-005

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