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Srivastav A
Srivastav A
Department of Microbial Gene Technology, School of Biological Sciences,
Madurai, Tamilnadu
India
Publications
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Research Article
Screening of Antimicrobial Activity and Polyketide Synthase Gene Identification from the Actinomycetes Isolates
Author(s): Srivastav ASrivastav A
The Streptomyces genus is well studied owing to its capacity in producing more than 70% of antibiotics. This study was undertaken to characterize Streptomyces strains, occurring in soils of different district of Tamilnadu, India as well as to evaluate their potential to produce antimicrobial compounds. Samples were collected from rice rhizosphere from different district of Tamilnadu agricultural zone. In primary screening by cross streak method, Streptomyces strains were assessed for antibiotic production and activity against different human bacterial. Then active isolates were selected for secondary screening by agar well diffusion method. Solvent extraction method was used to identify the best crude samples which are exhibiting better antibacterial activity. Then, 16S rRNA PCR was carried out for confirmation of isolates. In primary screening among all of .. View More»
DOI: 10.4172/1948-5948.1000404