Journal of Plant Pathology & Microbiology

Songbai Zhang

Publications

• Research Article
  Development of a Real-time RT-PCR Method for Rapid Detection and Quantification of Southern Rice Black-streaked Dwarf Virus in Rice
  Author(s): Songbai Zhang, Deyong Zhang, Yong Liu, Xiangwen Luo, Jue Cheng and Jing PengSongbai Zhang, Deyong Zhang, Yong Liu, Xiangwen Luo, Jue Cheng and Jing Peng
  
  Southern Rice Black-Streaked Dwarf Virus (SRBSDV) was a new pathogen species in genus Fijivirus group 2, which caused serious yield loss of many important cereal crops, such as rice and maize, in the past four years (2008-2011), which are imported in China and Southeast Asia. Notably, there are no typical symptoms in early infected rice, which can prevent serious yield loss. So, it is very important for detection of the early stage of infection of SRBSDV in rice. To this end, a real-time RT-PCR method was established and used to detect this virus in rice samples, and it was also compared with the conventional RT-PCR. The result showed that the real-time RT-PCR possessed high specificity and sensitivity for SRBSDV detecting, and the relationship between Ct values and copy number of SRBSDV was linear with a range of 4.04×102-4.04×107 copies/rea.. View More»
  DOI: 10.4172/2157-7471.1000187

  Abstract PDF