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Soler EP
Soler EP
Spain
Publications
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Research Article
A Comparative Study on Surface Treatments in the Immobilization Improvement of Hexahistidine-tagged Protein on the Indium Tin Oxide Surface
Author(s): Manel Ben Ismail, Pastor NC, Soler EP, Soltani A and Othmane AManel Ben Ismail, Pastor NC, Soler EP, Soltani A and Othmane A
Biosensors are becoming increasingly important in various sectors. However, choosing transducer type and surface treatment still faces severe limitations. Indium-tin oxide is widely used in electrochemical sensing, though surface cleaning and functionalization are not always straightforward and often poorly controlled. Here, we have covalently immobilized a hexahistidine-tagged model protein on three different treated ITO surfaces using a Ni2+ chelator moiety. The study shows that compared to two other treatments (H2O/H2O2/NH4OH and KOH treatments), the ITO Piranha treatment gives satisfactory results in term of yields of EC12 protein immobilized on the surface. The study in detail of treatments effect was realized by applying a combination of local and global techniques such X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), Attenuated.. View More»
DOI: 10.4172/2157-7439.1000372