Biochemistry & Analytical Biochemistry

Soboleva SE

Publications

• Research Article
  How Human Alpha-lactalbumin Recognize DNA and RNA
  Author(s): Nevinsky GA, Alinovskaya LI, Ivanisenko NV, Soboleva SE and Sedykh SENevinsky GA, Alinovskaya LI, Ivanisenko NV, Soboleva SE and Sedykh SE
  
  Human α-lactalbumin (LA) has an important function in mammary cells, activates the caspases involving in apoptosis. LA complex interacts with DNA in tumor cells with histones and impaired the chromatin structure. There are no any data on how LA recognizes DNA and interact with histones and DNA of chromatin. The approach of stepwise increase in ligand complexity was used for estimation of the relative contribution of every DNA nucleotide unit to its total affinity for human LA. It was shown that the LA DNA-binding site minimal ligands are orthophosphate and all dNMPs and rNMPs (Kd=(5.0-43)×10-5). Maximal contribution to the total affinity was observed for three nucleotide units of all (pN)n with a significant decrease in the order 1>2>3, at n=4-6 it was remarkably lower and at n ≥ 6-7 all dependencies of -logKd upon n reached plateaus. Double-stranded (pN)n showed.. View More»
  DOI: 10.4172/2161-1009.1000363

  Abstract PDF