Journal of Microbial & Biochemical Technology

Sima Rafati

Publications

• Research Article
  Assessment of Protection Induced by DNA and Live Vaccine Encoding Leishmania MHC Class I Restricted Epitopes against L. major Challenge in Balb/c Mice Model
  Author(s): Mojgan Zandieh, Tahereh Kashi, Tahereh Taheri, Farnaz Zahedifard, Yasaman Taslimi, Mahnaz Doustdary, Sima Habibzadeh, Ali Eslamifar, Fazel Shokri, Sima Rafati and Negar Seyed Mojgan Zandieh, Tahereh Kashi, Tahereh Taheri, Farnaz Zahedifard, Yasaman Taslimi, Mahnaz Doustdary, Sima Habibzadeh, Ali Eslamifar, Fazel Shokri, Sima Rafati and Negar Seyed
  
  Leishmaniasis is a neglected tropical disease endemic in more than 88 countries and is spreading over and over mainly due to transmigration and drug resistance. Unfortunately, despite increasing incidence, effective vaccine is still lagging behind. Unraveling CD8+ T-cells’ contribution in infection control has recently led a new concept into Leishmania vaccine research field as T-cell vaccine. Therefore we studied the efficacy of a CD8 stimulating T-cell vaccine (a string of beads) in two distinctive approaches as homologous DNA-DNA or heterologous DNALive prime-boost strategies. Here we hypothesized that CD8+ T-cell stimulation by polytope constructs diverts primary Th2 responses into Th1 resulting in disease control. Four recently reported H-2Kd restricted epitopes from proteins out of Leishmania vaccine candidate repertoire were included in the polytope construct (besides 13 .. View More»
  DOI: 10.4172/1948-5948.1000250

  Abstract PDF