Journal of Nanomedicine & Nanotechnology

Shiva K. Rastogi

Publications

• Research Article
  Antibody@Silica Coated Iron Oxide Nanoparticles: Synthesis, Capture of E.coli and Sers Titration of Biomolecules with Antibacterial Silver Colloid
  Author(s): Shiva K. Rastogi, Jamie M. F. Jabal, Huijin Zhang, Charlene M. Gibson, Kevin J. Haler, You Qiang, D. Eric Aston and A. Larry BranenShiva K. Rastogi, Jamie M. F. Jabal, Huijin Zhang, Charlene M. Gibson, Kevin J. Haler, You Qiang, D. Eric Aston and A. Larry Branen
  
  Silica coated iron oxide (SiO 2 /Fe 3 O 4 + γ- Fe 2 O 3 ) nanoparticles (SIO-NPs; 75±10 nm in diameter) were prepared by encapsulation of iron oxide NPs with silica using sol-gel method and characterized through spectroscopy methods. The SIO NPs were chemically activated by cyanogens bromide and then functionalized with Escherichia coli ( E. coli ) antibodies. These immuno-magnetic (IM NPs) were used to capture and concentrate E. coli from ~ 180 cfu/ mL suspension. The identification of bacteria was performed by plating on nutrient agar, fluorescent microscopy and scanning electron microscopy. Surface enhanced Raman spectroscopy (SERS) was used to identify different biomolecules of bacterial cell upon the interaction of colloidal silver nanoparticle (Ag NPs 6±4 nm) at different period of time. I.. View More»
  

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