Journal of Plant Pathology & Microbiology

Shahid Afghan

Publications

• Research Article
  Comparison of DAC-ELISA and Tissue Blot Immunoassay for the Detection of Acidovorax avenae subsp. avenae, causal agent of Red Strip of Sugarcane
  Author(s): Syed Zia-Ul-Hussnain, Rauf CA, Haque MI, Shahid Afghan, Tariq Mukhtar, Farah Naz, Kausar Nawaz Shah M and Aamir ShahazadSyed Zia-Ul-Hussnain, Rauf CA, Haque MI, Shahid Afghan, Tariq Mukhtar, Farah Naz, Kausar Nawaz Shah M and Aamir Shahazad
  
  Direct Antibody Coating Enzyme Linked Immunoassay (DAC-ELISA) and Tissue Blot Immunoassay (TBIA) were compared for detection of Acidovorax avenae subsp. avenae (Aaa) in sugarcane samples surveyed in 2010 and 2011. A total of 27 sugarcane clones contained two samples of symptomatic plants, which were tested simultaneously by both serological tests. The results obtained from DAC-ELISA tests showed that 11 of 27 tested samples of sugarcane clones were infected with Aaa and 16 were bacteria free; whereas, in duplicate samples only 7 samples were positive for Aaa using the TBIA. The TBIA membranes showed dark blue stain colour in the 7 positive and 20 were negative. It was concluded that the DAC-ELISA had false positives in the 4 clones (SPF-238, CP77-400, SPF-213 and GT-11) that were negative using TBIA. Assay time was also minimized in performing TBIA test as equivalence to DAC-ELISA. Re.. View More»
  DOI: 10.4172/2157-7471.1000172

  Abstract PDF