Journal of Bioequivalence & Bioavailability

Sekar Rajan

Publications

• Research Article
  Determination of Doxycycline in Human Plasma by Liquid Chromatography-Mass Spectrometry after Liquid-Liquid Extraction and its Application in Human Pharmacokinetics Studies
  Author(s): Muralidharan Selvadurai, Subramania Nainar Meyyanathan, Sekar Rajan, Govindarajan Padmanaban and Bhojraj SureshMuralidharan Selvadurai, Subramania Nainar Meyyanathan, Sekar Rajan, Govindarajan Padmanaban and Bhojraj Suresh
  
  A sensitive and reproducible liquid chromatography–mass spectrometric (LC-MS) method has been developed and validated for the quantifi cation of doxycycline in human plasma, after liquid-liquid extraction (LLE). Best chromatographic resolution was achieved on a reverse-phase Phenomenex C18 column using the mobile phase acetonitrile–5mM ammonium acetate (80:20) in isocratic elution with a total run time of 3.5 min. The analyte, doxycycline was detected by using an electrospray ionization mass spectrometer in the selected ion monitoring (SIM) mode. Linear plot was obtained in the concentration range of 0.5–5.0 µg/ml (r2 = 0.998). Lower limit of quantifi cation (LLOQ) was found to be 100 ng/ml in 500µl of plasma. Average recovery of the analyte was found to range from 96.08 to 104.60% in plasma at the concentrations of 1.0, 3.0 and 5.0 µg/ml. Intra- an.. View More»
  DOI: 10.4172/jbb.1000038

  Abstract PDF