Journal of Vaccines & Vaccination

Sérgio O De Paula

Publications

• Research Article
  Enhancement of Dengue-2 E Protein Expression by the Expression of the Precursor Membrane Protein (Prm) of the Dengue-3 Virus
  Author(s): Michelle D de Oliveira, André S de Oliveira, Nina R Dutra, Rafael F O França, Eduardo R Honda, Fernando B Zanchi, Clovis A Neves, Cynthia C da Silva, Benedito A L da Fonseca and Sérgio O De PaulaMichelle D de Oliveira, André S de Oliveira, Nina R Dutra, Rafael F O França, Eduardo R Honda, Fernando B Zanchi, Clovis A Neves, Cynthia C da Silva, Benedito A L da Fonseca and Sérgio O De Paula
  
  Several attempts to develop dengue recombinant subunit vaccines have failed due to insufficient levels of expression and incorrect folding of the E protein. In order to verify the importance of the precursor membrane protein to the level of E protein expression, we constructed two recombinant plasmids by cloning the full-length sequence of the prM gene from the dengue-2 and dengue-3 virus strains into a plasmid that expresses a truncated version of the E protein of the dengue-2 virus. Next, we evaluated these two constructs for their effect on the levels of E protein expression in vitro. Our results showed that both plasmids provided a correct expression of the E protein as E protein expression was detected in transfected Vero cells as demonstrated by indirect immunofluorescence and immunoblotting. Densitometry analysis of western blots of the cell extracts showed a 67.02% higher expr.. View More»
  DOI: 10.4172/2157-7560.1000182

  Abstract PDF