Journal of Nanomedicine & Nanotechnology

Saikat Talapatra

Publications

• Research Article
  Assembly of a Dual Aptamer Gold Nanoparticle Conjugate Ensemble in the Specific Detection of Thrombin when Coupled with Dynamic Light Scattering Spectroscopy
  Author(s): Vishala S Ramyah Aaryasomayajula, Tiffany Severs, Kartik Ghosh, Robert DeLong, Xianfeng Zhang, Saikat Talapatra and Adam K WanekayaVishala S Ramyah Aaryasomayajula, Tiffany Severs, Kartik Ghosh, Robert DeLong, Xianfeng Zhang, Saikat Talapatra and Adam K Wanekaya
  
  We demonstrate an extremely facile, rapid, specific and selective method for detecting proteins using aptamerconjugated gold nanoparticles coupled with dynamic light scattering (DLS) at ambient conditions. Addition of proteins to aptamer-conjugated gold nanoparticles (GNPs) induced the formation of protein-aptamer-gold nanoparticle conjugate complexes. The average hydrodynamic diameter of the nanoconjugate complexes as measured by DLS, increased with the corresponding increase in protein concentration. This correlation formed the analytical basis of the assay. The nanoparticles and nanoconjugate complexes were characterized by transmission electron microscopy, ultra-violet visible spectroscopy and DLS. Various parameters affecting the assay were optimized. Using thrombin as the model analyte, we demonstrated the detection of as low as 1.41 nM (0.05 μg/mL) of the protein. A linear d.. View More»
  DOI: 10.4172/2157-7439.1000210

  Abstract PDF