Journal of Plant Pathology & Microbiology

Saeedeh Aghaei

Publications

• Research Article
  Development and Application of Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Fusarium Oxysporum f. Sp. lycopersici
  Author(s): Mohammad Amin Almasi, Seyed Mohammad Hosseini Dehabadi, Aboubakr Moradi, Zahra Eftekhari, Mehdi Aghapour Ojaghkandi and Saeedeh AghaeiMohammad Amin Almasi, Seyed Mohammad Hosseini Dehabadi, Aboubakr Moradi, Zahra Eftekhari, Mehdi Aghapour Ojaghkandi and Saeedeh Aghaei
  
  A reliable and rapid pathogen detection protocol, for the first time, that utilizes colorimetric loop-mediated isothermal amplification (LAMP) was developed for detection of Fusarium oxysporum f. sp. lycopersici. In this regard, all six LAMP primers (i.e. F3, B3, FIP, BIP, LF and LB), together with PCR primers (F and R) were designed on the basis of the 28s ribosomal RNA gene (GenBank accession number: HM057281.1) of the fungi genome. Even though PCR and LAMP assays could successfully detect positive infected samples, considering the time, safety, cost and simplicity, the latter was overall superior. Furthermore, the results demonstrated that the LAMP assay was 100-fold sensitive and 4-fold faster compared to PCR. Interestingly, LAMP reaction could successfully detect Fusarium oxysporum f. sp. lycopersici without DNA purification (direct-LAMP). Meanwhile, among six visual dyes used to.. View More»
  DOI: 10.4172/2157-7471.1000177

  Abstract PDF