Pharmaceutica Analytica Acta

Saeed Arayne M

Publications

• Research Article
  A Validated Reverse Phase Liquid Chromatographic Method for Simultaneous Analysis of Enalapril Maleate, Hydrochlorothiazide and Furosemide in Active Pharmaceutical Ingredients, Pharmaceutical Dosage Forms and Human Serum
  Author(s): Saeed Arayne M, Safila Naveed and Najma Sultana Saeed Arayne M, Safila Naveed and Najma Sultana
  
  A sensitive, reproducible isocratic reversed phase method has been developed and validated for the simultaneous quantification of enalapril and diuretics (hydrochlorothiazide and furosemide) in active pharmaceutical ingredients, dosage formulations and human serum using high-performance liquid chromatography (RP-HPLC). The method was validated according to ICH guidelines for the parameters: specificity, stability, limits of detection (LLOD), limits of quantification (LLOQ), linearity, accuracy, precision and recovery. Chromatographic separation was performed on Hypersil ODS C18 (150×4.6mm, 5micron) and Purospher Start C18 (250 mm×4.6 mm, 5 μm) columns using gradient elution, when methanol: water (75:25 v/v) was used as the mobile phase and pH adjusted to 3 with orthophosphoric acid having flow rate of 1.0 mL min-1 at ambient temperature. The lower limit of quantitation .. View More»
  DOI: 10.4172/2153-2435.1000244

  Abstract PDF