Pharmaceutica Analytica Acta

Saeed Arayne M

Publications

• Research Article
  Simultaneous Liquid Chromatographic Determination of Two Co- Prescribed Anti-Cancer Drugs in Bulk Drug, Dosage Formulations and in Human Serum Using Multivariate Technique: Application to in vitro Drug Interaction
  Author(s): Najma Sultana, Saeed Arayne M, Saeeda Nadir Ali and Arman Tabassum Najma Sultana, Saeed Arayne M, Saeeda Nadir Ali and Arman Tabassum
  
  Present study describes the development and validation of a high-performance liquid chromatographic (HPLC) assay for the simultaneous quantification of atorvastatin and celecoxib in human serum and pharmaceutical formulations. The separation was carried out on Bondapak, C18 (10 ?m, 25×0.46 cm) column using mobile phase 80:20 methanol-water of pH 3.5, maintained with o-phosphoric acid. The method was optimized using multivariate technique and detector response was recorded at five different wavelengths. Chromatography was performed at ambient temperature by pumping the mobile phase at a flow rate of 1.0 mL min-1. Calibration curves were found to be linear with correlation coefficient greater than 0.998 over the concentration range 2-60 and 2-70 ng mL-1 with detection limits 0.024 and 0.019 ng mL-1 in bulk drug and 0.04 and 0.01 ng mL-1 in serum at isobestic point. The method was validated.. View More»
  DOI: 10.4172/2153-2435.1000215

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