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Saeed Ahmad
Saeed Ahmad
Pakistan
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Research
Comparative Study of PCR and ELIZA for the Detection of Foot and Mouth Disease in Cattle
Author(s): Abdul Sajid, Natasha Kashif, Muhammad Bilal, Mian Muhammad Salman, Saeed Ahmad and Jun Li Abdul Sajid, Natasha Kashif, Muhammad Bilal, Mian Muhammad Salman, Saeed Ahmad and Jun Li
Among viral diseases of animals, Foot and Mouth Disease is an exceedingly transferable disease of clovenhoofed animals caused by the virus of class Aphthovirus, family Picornaviridae. In the present study the Indirect Sandwich ELISA negative samples were tested through PCR and a range of OD for false negative ELISA samples was adjusted in order to decide whether the samples are actually positive or negative. Total 50 ELISA’s negative samples were confirmed through PCR. Out of total 50 ELISA’s negative samples, 23 were positive for PCR while rests of the 27 samples were found negative. In the PCR positive samples 13, 2 and 3 were positive for Serotype “O”, “A” and “Asia-1”, respectively. While 2 samples were positive for O and A and 3 for O and Asia-1 mixed infection. On the basis of type of samples, it was found that 18.. View More»
DOI: 10.4172/2157-7560.1000382