Journal of Aquaculture Research & Development

S. Barkataki

Publications

• Research Article
  Cortisol Inhibition of 17b-Estradiol Secretion by Rainbow Trout Ovarian Follicles Involves Modulation of Star and P450scc Gene Expression
  Author(s): S. Barkataki, N. Aluru, M. Li, L. Lin, H. Christie, M.M. Vijayan and J.F. Leatherland S. Barkataki, N. Aluru, M. Li, L. Lin, H. Christie, M.M. Vijayan and J.F. Leatherland
  
  Maternal stress associated with elevated maternal cortisol levels has well-demonstrated adverse affects on reproduction in vertebrates, including impaired ovarian steroidogenesis. Currently, the potential sites of action of cortisol on ovarian function are not known. Studies were carried out to examine the mechanism(s) by which cortisol suppresses steroidogenesis by mid-vitellogenic stage rainbow trout (Oncorhynchus mykiss) ovarian follicles. 17b-Estradiol and testosterone synthesis, and the expression of key steroidogenesis-related genes (using real time RT-PCR) were measured. Follicles were also incubated in the presence of several tritium-labelled steroids ([3H]17α-hydroxyprogesterone, [3H]testosterone, [3H]androstenedione), and the tritium-labeled steroid products were separated by reverse-phase HPLC to look for possible affects of cortisol .. View More»
  DOI: 10.4172/2155-9546.S2-001

  Abstract PDF