Journal of Bioequivalence & Bioavailability

Ruichen Guo

Publications

• Research Article
  Determination of Uridine in Human Plasma by HPLC and its Application in Citicoline Sodium Pharmacokinetics and Bioequivalence Studies
  Author(s): Keguang Chen, Xiaoyan Liu, Chunmin Wei, Guiyan Yuan, Rui Zhang, Rong Li, Benjie Wang and Ruichen GuoKeguang Chen, Xiaoyan Liu, Chunmin Wei, Guiyan Yuan, Rui Zhang, Rong Li, Benjie Wang and Ruichen Guo
  
  A simple and rapid high performance liquid chromatography (HPLC) method was developed and validated to determine uridine, metabolite of citicoline, in human plasma, and applied to the pharmacokinetics and bioequivalence studies of citicoline sodium tablet and capsule. Uridine and amoxicillin (used as internal standard, IS) were extracted from plasma by simple protein precipitation with perchloric acid. The separation was performed on a Phenomenex kinetex C18 (100 ×4.6 mm, 2.6μm) column, with a mobile phase of 0.05 M phosphate buffer (Potassium dihydrogen phosphate, adjusted pH to 3.5 by phosphate) - Methanol (98:2, V/V), and delivered at the flow rate of 0.8 mL/min. The calibration curve was linear over uridine concentration range of 0.05-2μg/mL (r>0.99), The main pharmacokinetic parameters of citicoline sodium tablet and capsule were as follows, t 1/2 (4.393±2.5.. View More»
  DOI: 10.4172/jbb.1000062

  Abstract PDF