Journal of Nanomedicine & Nanotechnology

Roxana V. Alasino

Publications

• Research Article
  Selective Binding of Albumin to Gm1 Ganglioside Micelles Containing Paclitaxel
  Author(s): Victoria Leonhard, Roxana V. Alasino, Ismael D. Bianco and Dante M. BeltramoVictoria Leonhard, Roxana V. Alasino, Ismael D. Bianco and Dante M. Beltramo
  
  In our previous work we showed that taxanes (paclitaxel (Ptx) and docetaxel(Dtx)) can be spontaneously loaded into monosialoganglioside (GM1) nanomicelles, increasing their water solubility about 6,000 times, to render stable water soluble formulations that could be used as a novel strategy to deliver drugs in cancer. Here, we describe the hydrophobic interaction of Human Serum Albumin (HSA) with GM1 micelles loaded with Ptx, as a strategy that could improve tumour drug accumulation. This interaction is regulated by conditions such pH and temperature and generates ternary complexes GM1/Ptx/HSA with sizes around 19 to 24 nm and hydrodynamic radius equivalent to a globular protein of 140-180 kDa. These mixed micelles were stable in solution for at least 40 days and also upon freeze-thawing or lyophilization-solubilization cycles. The results of in vitro assays showed that the nano-struc.. View More»
  DOI: 10.4172/2157-7439.1000159

  Abstract PDF