Rouhani Nejad H
West Indies
Research
Molecular Detection of Enterotoxin B from Staphylococcus aureus
Author(s): Sheikhi F, Zeinoddini M*, Saeedinia AR, Rouhani Nejad H and Monazah A
Sheikhi F, Zeinoddini M*, Saeedinia AR, Rouhani Nejad H and Monazah A
Background: Food poisoning due to the bacteria is a big global problem in economically and human's health. This problem refers to an illness which is due to infection or the toxin exists in nature and the food that we use. One of the most important productive factors is Staphylococcus aureus. In general different strain of S. aureus produces several enterotoxins like: SEA, SEB, SEC. So, the existence of S. aureus in foods is a potential risk, especially if the foods are not storage in proper temperature. In this work, by designing of new primer the enterotoxin B (SEB) has been detected using PCR techniques.
Material and method: In this study, the primer explorer V4 software was used for designing specific primers. The extracted DNA genomic from S. aureus was using for PCR reaction. The PCR product anal.. View More»
DOI:
10.4172/2576-389X.1000117