Journal of Microbial & Biochemical Technology

Rolf D

Publications

• Research Article
  Genes that are Affected in High Hydrostatic Pressure Treatments in a Listeria Monocytogenes Scott A ctsR Deletion Mutant
  Author(s): Yanhong Liu, Lihan Huang, Rolf D, Joerger and Nereus W. Gunther IVYanhong Liu, Lihan Huang, Rolf D, Joerger and Nereus W. Gunther IV
  
  Listeria monocytogenes is a food-borne pathogen of significant threat to public health. High Hydrostatic Pressure (HHP) treatment can be used to control L. monocytogenes in food. The CtsR (class three stress gene repressor) protein negatively regulates the expression of class III heat shock genes. In a previous study, a spontaneous ctsR L. monocytogenes deletion mutant 2-1 that was able to survive under HHP treatment was identified; however, there is only limited information about the mechanisms of survival and adaptation of this mutant in response to high pressure. Microarray technology was used to monitor the gene expression profiles of ctsR mutant 2-1 under pressure treatments (450 Mpa, 3min). Some of the gene expression changes determined by microarray assays were confirmed by real-time RT-PCR analyses. Compared to non-pressure-treated ctsR mutant 2-1, 14 genes were induced (> .. View More»
  DOI: 10.4172/1948-5948.S2-003

  Abstract PDF