Journal of Bioequivalence & Bioavailability

Rogério A. Oliveira

Publications

• Research Article
  Comparative Bioavailability and Pharmacodynamic Aspects of Cyclobenzaprine and Caffeine in Healthy Subjects and the Effect on Drowsiness Intensity
  Author(s): Ronilson A. Moreno, Carlos Eduardo Sverdloff, Rogério A. Oliveira, Sandro Evandir Oliveira, Diego Carter Borges, Maristela H. Andraus, Myriam C. Salvadori and Ney Carter BorgesRonilson A. Moreno, Carlos Eduardo Sverdloff, Rogério A. Oliveira, Sandro Evandir Oliveira, Diego Carter Borges, Maristela H. Andraus, Myriam C. Salvadori and Ney Carter Borges
  
  A specific, fast and sensitive LC–MS/MS assay was d e- veloped for the determination of cyclobenzaprine in hu- man plasma using imipramine as the internal standar d (IS). The limit of quantification was 0.05 ng/mL and the method was linear in the range of 0.05 to 50 ng/mL. The cyclobenzaprine and IS retention times were 2.74±0. 2 min and 2.69±0.2 min, respectively. Method intra-batch precision and accuracy ranged fr om 2.90 to 9.72%, and 91.63 to 107.33%, respectively. Inter- batch precision ranged from 3.37 to 10.27%, while I nter- batch accuracy ranged from 96.13 to 106.10%. The an a- lytical method was applied to evaluate the pharmaco ki- netic and relative bioavailability of two different pharma- ceutical formulations containing cyclobenzaprine, o ne test tablet containing 10 mg of cyclobenzaprine plus 60 mg of caffeine (Miosan ® /cafeine) and the reference.. View More»
  DOI: 10.4172/jbb.1000013

  Abstract PDF