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Robert L. Schlub
Robert L. Schlub
Tanzania
Publications
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Research Article
Specific Detection of Klebsiella variicola and K. oxytoca by Loop-Mediated Isothermal Amplification
Author(s): Jarred Yasuhara-Bell, Caleb Ayin, April Hatada, Yonghoon Yoo, Robert L. Schlub and Anne M. Alvarez Jarred Yasuhara-Bell, Caleb Ayin, April Hatada, Yonghoon Yoo, Robert L. Schlub and Anne M. Alvarez
Klebsiella spp. are opportunistic pathogens with clinical, veterinary and plant-associated isolates. A previous study showed that bacterial ooze from wetwood of severely declined ironwood trees in Guam contained Ralstonia solanacearum, Klebsiella variicola and K. oxytoca. In this study, Loop-Mediated Isothermal Amplification (LAMP) detected K. variicola and K. oxytoca specifically, using unique primer sets designed individually for each organism. Each LAMP detected its target specifically, while showing negative results for non-target bacteria and negative controls. LAMP detected Klebsiella in inoculated-ironwood stem tissues and bacterial ooze. Due to the presence of plant inhibitors, different sampling protocols were tested. Soaking plant tissue samples to allow diffusion of bacteria into solution, followed by boiling, provided optimum detection of .. View More»
DOI: 10.4172/2157-7471.1000271