Journal of Stem Cell Research & Therapy

Renato Romagnoli

Publications

• Research Article
  The Angiogenic Potential of Adipose Mesenchymal Stem Cell-derived Extracellular Vesicles is modulated by Basic Fibroblast Growth Factor
  Author(s): Tatiana Lopatina, Aurora Mazzeo, Stefania Bruno, Ciro Tetta, Natalia Kalinina, Renato Romagnoli, Mauro Salizzoni, Massimo Porta and Giovanni Camussi Tatiana Lopatina, Aurora Mazzeo, Stefania Bruno, Ciro Tetta, Natalia Kalinina, Renato Romagnoli, Mauro Salizzoni, Massimo Porta and Giovanni Camussi
  
  Objective: The aim of the present study was to investigate the effects of basic fibroblast growth factor (bFGF) on the angiogenic properties of extracellular vesicles (EVs) secreted by adipose-derived mesenchymal stem cells (ASCs) in culture. Methods: We isolated EVs from ASC (ASC-EVs) cultured with bFGF (FGF-EVs) or without bFGF (b-EVs). We compared the EV angiogenic action on human microvascular endothelial cells (HMEC) in vitro by capillarylike structure formation assay and in vivo by subcutaneous injection of HMEC-containing Matrigel in SCID mice. We analysed micro-RNA composition of EVs by PCR array and we selected two mi-RNAs changed after bFGF stimulation: miR-223 and miR-21. We transfected HMEC with short antisense anti-miR-223 or with mimic miR- 21 to compensate the action of EVs. Result.. View More»
  DOI: 10.4172/2157-7633.1000245

  Abstract PDF