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Rastko Joksimovic
Rastko Joksimovic
Germany
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Research Article
Size and Cell Type Dependent Uptake of Silica Nanoparticles
Author(s): I-Lun Hsiao, Annika Mareike Gramatke, Rastko Joksimovic, Marek Sokolowski, Michael Gradzielski and Andrea HaaseI-Lun Hsiao, Annika Mareike Gramatke, Rastko Joksimovic, Marek Sokolowski, Michael Gradzielski and Andrea Haase
As silica nanoparticles (SiO2 NP) gain increasing interest for medical applications it is important to understand their potential adverse effects for humans. Here we prepared well-defined core-shell fluorescently labelled SiO2 NP of 15, 60 and 200 nm diameter and analyzed their cytotoxicity in THP-1 derived macrophages, A549 epithelial cells, HaCaT keratinocytes and NRK-52E kidney cells. We observed a size-dependent cytotoxicity in all cell types in serumfree conditions. HaCaT cells were least and macrophages or lung derived A549 cells were highly sensitive towards SiO2 NP treatment. Differences in cytotoxicity could be correlated with different uptake rates. By using flow cytometry and confocal microscopy we quantified the uptake. Furthermore we used specific inhibitors for clathrin- and caveolinmediated endocytosis to elucidate the uptake mechanisms, whi.. View More»
DOI: 10.4172/2157-7439.1000248