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Rao M
Rao M
Italy
Publications
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Research Article
Development and Validation of a New Ultra-fast HPLC Method for Quantification of Levofloxacin in Rabbit Aqueous Humour: Application to a Pharmacokinetic Study
Author(s): Spadaro A, Lorenti M, Zasa G and Rao M Spadaro A, Lorenti M, Zasa G and Rao M
A specific, sensitive, reliable, low-cost, isocratic, ultra-fast HPLC-DAD for Levofloxacin quantification in rabbit aqueous humor was developed, validated and applied to a pharmacokinetic study, conducted on commercial formulation of Levofloxacin. Separations were obtained on a XB-C18 column (100A, 100 mm × 4.60 mm, 2.6 μm, Phenomenex) with an isocratic mobile phase consisting of 18% acetonitrile and 82% triethylamine 0.5% in water (pH adjusted to 2.5 with H3PO4) at a flow rate of 0.5 ml/min. Detection of levofloxacin was done at 292 nm, and the column temperature was 40°C. The total analysis run-time was 5 min per sample, and no interfering peaks from aqueous humor were detected. Method was found to be selective, linear (R2=0.99984), accurate (intra-day recovery, 98.24%-100.04%) and precise (RSD, ≤ 5.50%). Pharmacokinetic parameters in rabbit aqueous humors were calcu.. View More»
DOI: 10.4172/2153-2435.1000603