Rania Aydi Ben Abdallah
Tunisia
Research Article
Effect of Temperatures and Culture Media on Sclerotium rolfsii Mycelial Growth, Sclerotial Formation and Germination
Author(s): Fakher Ayed, Hayfa Jabnoun-Khiareddine, Rania Aydi Ben Abdallah and Mejda Daami-RemadiFakher Ayed, Hayfa Jabnoun-Khiareddine, Rania Aydi Ben Abdallah and Mejda Daami-Remadi
Sclerotium rolfsii is a serious soilborne phytopathogenic fungus causing serious yield loss in crops of high economic importance. In this study, the effects of temperatures and culture media were evaluated on S. rolfsii mycelial growth, sclerotial production and germination. The three isolates tested grew over a range of temperatures from 10°C to 35°C but not at 5°C and 40°C. Radial growth and dry mycelium production were highest at 30°C. Sclerotial initiation started on the 3rd day after incubation at 30°C and 35°C, and mature sclerotia were observed after 15 and 6 days of incubation, respectively. Optimal sclerotial production and the dry weight of 100 sclerotia varied depending on isolates and temperatures and occurred at 25°C-35°C. No mature sclerotia were produced at 5°C, 10°C, 15°C and 40°C. Sclerotial germination, noted after .. View More»
DOI:
10.4172/2157-7471.1000446