Journal of Microbial & Biochemical Technology

Ramya S

Publications

• Research Article
  Diverse Pattern of Cytokine Production, their Functional Association and mRNA Expression in Tuberculosis Patients with Diabetes Mellitus and their Household Contacts
  Author(s): Meenakshi P, Ramya S, Madhavi Latha A, Lavanya J and Sumanlatha GMeenakshi P, Ramya S, Madhavi Latha A, Lavanya J and Sumanlatha G
  
  Cytokine genes are associated with their altered production and play a significant role in tuberculosis patients with diabetes mellitus and their household contacts. We aimed to study the influence of IL-1β and IL-6 gene polymorphisms on cytokine gene production and to evaluate their mRNA expression in M.tbAg85A stimulated culture supernatants of tuberculosis patients with diabetes mellitus, pulmonary tuberculosis patients, household contacts and diabetes individuals. Enzyme linked immunosorbent assay was used to estimate cytokine production in the culture supernatants and TRIZOL method for mRNA expression. Cytokine production was low in all the subjects when compared to the healthy controls and IL-6 levels increased with treatment. The CC genotype of IL-6 -174 G/C was associated with its low production in household contacts of tuberculosis patients with diabetes mellitus and pul.. View More»
  DOI: 10.4172/1948-5948.1000358

  Abstract PDF