Journal of Stem Cell Research & Therapy

Raf Malecki

Publications

• Research Article
  TRA-1-60+, SSEA-4+, POU5F1+, SOX2+, NANOG+ Clones of Pluripotent Stem Cells in the Embryonal Carcinomas of the Testes
  Author(s): Marek Malecki, Xenia Tombokan, Mark Anderson, Raf Malecki and Michael Beauchaine Marek Malecki, Xenia Tombokan, Mark Anderson, Raf Malecki and Michael Beauchaine
  
  Introduction: Cancer of the testes is currently the most frequent neoplasm and a leading cause of morbidity in men 15-35 years of age. Its incidence is increasing. Embryonal carcinoma is its most malignant form, which either may be resistant or may develop resistance to therapies, which results in relapses. Cancer stem cells are hypothesized to be drivers of these phenomena. Specific aim: The specific aim of this work was identification and isolation of spectra of single, living cancer stem cells, which were acquired directly from the patients’ biopsies, followed by testing of their pluripotency. Patients-methods: Biopsies were obtained from the patients with the clinical and histological diagnoses of the primary, pure embryonal carcinomas of the testes. Antibodies targeting TRA-1-60 and SSEA-4 were bioengineered to render magnetic or fluorescent. The cell surfa.. View More»
  DOI: 10.4172/2157-7633.1000134

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