Journal of Stem Cell Research & Therapy

Qin Li

Publications

• Research Article
  Development of Prevascularized Cell Sheets Using Single Bone Marrow Mesenchymal Stem Cell Source for Tissue Regeneration
  Author(s): Qin Li, Yuxia Feng, Dan Zhang, Jin Ren, Dongyang Ma and Liling RenQin Li, Yuxia Feng, Dan Zhang, Jin Ren, Dongyang Ma and Liling Ren
  
  Developing a vascular network for tissue engineering constructs is still a challenge. Cell-sheet technology has already brought promising potential in vascularization. However, endothelial cell source for vascularization is limited due to the difficulties in obtaining endothelial cells from peripheral blood or bone marrow. In this study, we differentiated rabbit bone marrow mesenchymal stem cells (rBMSCs) into endothelial like cells (ECs) by adding vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (b-FGF) into the culture medium of BMSCs. Then, the induced ECs were seeded on rBMSCs sheets to form vascularized ECs/BMSCs cell sheets. Results show that ECs on the undifferentiated BMSCs sheets formed networks in vitro and in vivo. In vivo results demonstrated that the quantity of vessels in the non-prevascularized constructs was lower than that of prevascularize.. View More»
  DOI: 10.4172/2157-7633.1000351

  Abstract PDF