Journal of Nanomedicine & Nanotechnology

Pierre Couleaud

Publications

• Research Article
  Functionalization of Iron Oxide Magnetic Nanoparticles with the Multivalent Pseudopeptide N6l for Breast Tumor Targeting
  Author(s): Maha Sader, Pierre Couleaud, Gilles Carpentier, Maud-Emmanuelle Gilles, Noureddine Bousserrhine, Alexandre Livet, Ilaria Cascone, Damien Destouches, Aitziber L Cortajarena and José Courty Maha Sader, Pierre Couleaud, Gilles Carpentier, Maud-Emmanuelle Gilles, Noureddine Bousserrhine, Alexandre Livet, Ilaria Cascone, Damien Destouches, Aitziber L Cortajarena and José Courty
  
  Functionalized iron oxide magnetic nanoparticles (MNP) are an innovative tool for cancer detection and treatment. In this study, a cell-surface nucleolin antagonist, N6L, was used as targeting ligand for MNP. This N6L, which exhibits antitumor activities, specifically targets bind tumor cells by binding to cell-surface nucleolin and glycosaminoglycans. N6L was covalently conjugated to dimercaptosuccinic acid coated magnetic nanoparticles (MNP-N6L). Using immunoprecipitation, gene invalidation and enzymatic degradation of glycosaminoglycans, we showed that MNP-N6L targets human breast cancer MDA-MB 231 cells through interaction with nucleolin and sulfated glycosaminoglycans. In vivo biodistribution studies were carried out in MDA-MB 231 tumor-bearing mice, using iron detection assay by spectrometric analysis and Prussian blue staining. Whereas both non-functionalized and functionalized.. View More»
  DOI: 10.4172/2157-7439.1000299

  Abstract PDF