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Pérez de Ciriza C
Pérez de Ciriza C
Spain
Publications
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Research Article
Macro Creatine Kinase: Comparison of Different Screening Methods and Revision of the Bibliography
Author(s): Pérez de Ciriza C and Nerea Varo Pérez de Ciriza C and Nerea Varo
Introduction: Macro-creatine kinase (Macro-CK) is a complex with longer half-life that leads to elevation of enzyme activity and analytical errors. We present two cases with macro-CK to picture the problem. The aim of the study was to evaluate and compare different methods using polyethylene glycol (PEG) for screening. Methods: Serum samples (n=39) were analysed using different PEG methods PEG6000, 30 minutes centrifugation, 3000 rpm, PEG8000, 10 minutes incubation and 5 minutes centrifugation, 1000 g Modified method 1, 10 minutes centrifugation. The recovery percentage and the polyethylene glycol precipitation activity (PPA) were calculated. In four patients macro-CK was confirmed by electrophoresis (Sebia). Results: The recovery percentage was significantly higher in all non-macro-CK samples than.. View More»
DOI: 10.4172/2161-1009.1000161