Journal of Bioequivalence & Bioavailability

Pavan Balabathula

Publications

• Research Article
  Rapid Quantitative Evaluation of Amphotericin B in Human Plasma, by Validated HPLC Method
  Author(s): Pavan Balabathula, Dileep R Janagam, Nivesh K Mittal, Bivash Mandal, Laura A Thoma and George C WoodPavan Balabathula, Dileep R Janagam, Nivesh K Mittal, Bivash Mandal, Laura A Thoma and George C Wood
  
  A simple, rapid, and sensitive HPLC method was developed and validated to quantify Amphotericin B (AmB) in human plasma. AmB was extracted from spiked plasma by simple protein precipitation with methanol. The separation was performed on an XBridge TM C18 (150 × 4.6 mm, 3.5 μm) column, with a mobile phase of acetic acid (0.73%) – acetonitrile (60:40, v/v) and at a flow rate of 1 mL/min. The eluted peak of AmB was monitored at 408 nm with photo-diode array detector (PDA) detector. The calibration curve was found linear in the AmB concentration range of 1000 – 50 ng/mL (r 2 >0.99). The inter- and intra-day precisions (%CV) were less than 11.2%. The extraction recoveries were 85-91%. The method developed and validated is simple, rapid (<3 min per injection), sensitive, and reproducible. It potentially can be used for the pharmacokinetic, bioequivalence, and toxi.. View More»
  DOI: 10.4172/jbb.1000145

  Abstract PDF