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Journal Flyer
Journal of Plant Pathology & Microbiology

Paul WJ Taylor

Paul WJ Taylor

Parkville 3010 VIC
Australia

Publications
  • Research Article
    The Role of Cutinase and its Impact on Pathogenicity of Colletotrichum truncatum
    Author(s): Adelene SM Auyong, Rebecca Ford and Paul WJ Taylor Adelene SM Auyong, Rebecca Ford and Paul WJ Taylor

    The phytopathogenic fungus, Colletotrichum truncatum infects and colonises chili fruit through direct penetration of the cuticle. The cutinase gene of C. truncatum (CtCut1), a cutin degrading enzyme was identified, cloned and shown to be essential in breaching the cuticle of chili fruit. The expression of CtCut1 gene was studied through RNA-mediated gene silencing and its impact on fungal pathogenicity was demonstrated. The vector, pAA1 encoding a hairpin RNA of GFP and CtCut1 was constructed and transformed into C. truncatum pathotype F8-3B (virulent strain). F8-3B-pAA1 transformants exhibited reduced patterns of infection with one isolate having a 45.8% reduction in cutinase activity (reduction in CtCut1 transcript) in comparison to the wild type. Importantly, CtCut1-deficient strains were unable to infect detached chili and soybean hosts as efficie.. View More»
    DOI: 10.4172/2157-7471.1000259

    Abstract PDF