Journal of Nanomedicine & Nanotechnology

Osvaldo Delbono

Publications

• Research Article
  Magnetofection Enhances Adenoviral Vector-based Gene Delivery in Skeletal Muscle Cells
  Author(s): Andrea Soledad Pereyra, Olga Mykhaylyk, Eugenia Falomir Lockhart, Jackson Richard Taylor, Osvaldo Delbono, Rodolfo Gustavo Goya, Christian Plank and Claudia Beatriz Hereñu Andrea Soledad Pereyra, Olga Mykhaylyk, Eugenia Falomir Lockhart, Jackson Richard Taylor, Osvaldo Delbono, Rodolfo Gustavo Goya, Christian Plank and Claudia Beatriz Hereñu
  
  The goal of magnetic field-assisted gene transfer is to enhance internalization of exogenous nucleic acids by association with magnetic nanoparticles (MNPs). This technique named magnetofection is particularly useful in difficultto- transfect cells. It is well known that human, mouse, and rat skeletal muscle cells suffer a maturation-dependent loss of susceptibility to Recombinant Adenoviral vector (RAd) uptake. In postnatal, fully differentiated myofibers, the expression of the primary Coxsackie and Adenoviral membrane receptor (CAR) is severely downregulated representing a main hurdle for the use of these vectors in gene transfer/therapy. Here we demonstrate that assembling of Recombinant Adenoviral vectors with suitable iron oxide MNPs into magneto-adenovectors (RAd-MNP) and further exposure to a gradient magnetic field enables to efficiently overcome transduction .. View More»
  DOI: 10.4172/2157-7439.1000364

  Abstract PDF